biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
canine, porcine, rabbit, mouse, human, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Cd44(281057)
dog ... Cd44(403939)
human ... CD44(960)
mouse ... Cd44(12505)
pig ... Cd44(100525150)
General description
Recognizes CD44v10 in endothelial cells.
Anti-CD44v10, rabbit polyclonal, recognizes CD44v10 in endothelial cells. It is validated for ELISA, FC, IP, RIA, WB, and IHC on frozen and paraffin sections.
Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) chromatography. Recognizes the CD44v10 protein.
Immunogen
Human
a synthetic peptide corresponding to amino acids from human CD44v10
Application
ELISA (1:2000)
Flow Cytometry (1:1000)
Frozen Sections (1:250)
Immunoblotting (1:10,000)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Paraffin Sections (1:250)
Radioimmunoassay (1:5000)
Flow Cytometry (1:1000)
Frozen Sections (1:250)
Immunoblotting (1:10,000)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Paraffin Sections (1:250)
Radioimmunoassay (1:5000)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Lokeshwar, V.B., et al. 1996. J. Biol. Chem.271, 23853.
Dall, P., et al. 1994. Cancer Res.54, 3337.
Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA89, 12160.
Arch, R., et al. 1992. Science275, 682.
Dall, P., et al. 1994. Cancer Res.54, 3337.
Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA89, 12160.
Arch, R., et al. 1992. Science275, 682.
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Steven J Wang et al.
The Laryngoscope, 119(8), 1518-1530 (2009-06-10)
The CD44 family of receptors includes multiple variant isoforms, several of which have been linked to malignant properties including migration, invasion, and metastasis. The objective of this study was to investigate the role of the CD44 v3, v6, and v10
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