Casein Kinase II, α-Subunit, Human, Recombinant, E. coli

Note: 1 mU = 1 milliunit.

Recombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

Recombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where the autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt sensitive with respect to its activity. Easily inactivated by treatment with heat, trypsin digestion, or urea.

Please refer to vial label for lot-specific concentration.

In 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.

Following initial thaw, aliquot and freeze (-70°C).

Allende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.

slight autoproteolysis yields one additional band of 39 kDa.however, no loss in activity is associated with the appearance of this 39 kDa product

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)