Caspase-5, Human, Recombinant, E. coli

M.W. 10,000 and 22,000 (heterodimer).

Recombinant, human caspase-5 expressed in E. coli. A member of the caspase-1 subfamily of cysteine proteases involved in Fas-mediated apoptosis. Caspase-5 cleavage site is reported to be Asp³¹¹ and Asp³³⁰. May also cleave at Asp¹²¹. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

After initial defrost, aliquot and freeze at -70°C.

Schwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.

One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805 ) per min at 25°C, pH 7.4.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)