A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).

Cell permeable: no

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

In DMSO.

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Irritant (B)