Diethylamine NONOate/AM

Cell permeable: yes

Primary Target
Intracellular nitric oxide (NO) donor

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 8.3 µM, 53 µM, acting as an antiproliferative agent in NO-sensitive human leukemia HL-60 and U937 cell lines, respectively

Toxicity: Standard Handling (A)

A cell-permeable O²-acetoxymethylated diazeniumdiolate that donates nitric oxide (NO) following the action of intracellular esterases (1.83 mol of NO/mol). Shown to induce apoptosis and act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC₅₀ = 8.3 µM) and U937 (IC₅₀ = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation (~5 µM) in bovine aortic endothelial cells. Useful for temporal and spatial controlled NO availability.

An esterase-sensitive and cell permeable, O²-acetoxymethylated diazeniumdiolate that acts as an intracellular nitric oxide (NO) donor (1.83 mol of NO/mol). Shown to induce apoptosis and to act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC₅₀ = 8.3 µM) and U937 (IC₅₀ = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation in bovine aortic endothelial cells. Exhibits enhanced stability in physiological buffers. May also be useful for temporal and spatial controlled NO availability.

Kotamraju, S., et al. 2001. J. Biol. Chem. 276, 17316.
Saavedra, J.E., et al. 2000. J. Med. Chem.43, 261.

Packaged under inert gas

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 2 months at -20°C. Add to aqueous medium just prior to use.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany