324390
Anti-Dopamine D1 Receptor Rabbit pAb
lyophilized, Calbiochem®
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, mouse, rat
Application:
—
Citations:
9
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended))
isotype
IgG
shipped in
ambient
Quality Level
Gene Information
human ... DRD1(1812)
rat ... Drd1(24316)
target post-translational modification
unmodified
General description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
Recognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.
Immunogen
Human
a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
Application
ELISA (see comments)
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended)
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended)
Physical form
Undiluted serum.
Preparation Note
Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Analysis Note
Positive Control
Rat brain
Rat brain
Other Notes
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Hye-Min Jeon et al.
Neoplasia (New York, N.Y.), 39, 100894-100894 (2023-03-28)
Recent studies indicate that signaling molecules traditionally associated with central nervous system function play critical roles in cancer. Dopamine receptor signaling is implicated in various cancers including glioblastoma (GBM) and it is a recognized therapeutic target, as evidenced by recent
Florian Giesert et al.
PloS one, 8(5), e63778-e63778 (2013-05-16)
Missense mutations in the leucine-rich repeat kinase 2 gene (LRRK2) are linked to autosomal dominant forms of Parkinson's disease (PD). In order to get insights into the physiological role of Lrrk2, we examined the distribution of Lrrk2 mRNA and different
Dana C Borcherding et al.
PloS one, 6(9), e25537-e25537 (2011-10-04)
Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective
Jacqueline S Coley et al.
PloS one, 10(2), e0117450-e0117450 (2015-02-04)
Drug abuse is a major comorbidity of HIV infection and cognitive disorders are often more severe in the drug abusing HIV infected population. CD14+CD16+ monocytes, a mature subpopulation of peripheral blood monocytes, are key mediators of HIV neuropathogenesis. Infected CD14+CD16+
Gabriela Assis-de-Lemos et al.
Bioscience reports, 41(12) (2021-11-26)
Dopamine signaling has numerous roles during brain development. In addition, alterations in dopamine signaling may be also involved in the pathophysiology of psychiatric disorders. Neurodevelopment is modulated in multiple steps by reactive oxygen species (ROS), byproducts of oxidative metabolism that
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