生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
lyophilized
不包含
preservative
种属反应性
human, mouse, rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
dilution
(ELISA
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended))
同位素/亚型
IgG
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... DRD1(1812)
rat ... Drd1(24316)
一般描述
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
Recognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.
免疫原
Human
a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
应用
ELISA (see comments)
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended)
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended)
外形
Undiluted serum.
制备说明
Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
分析说明
Positive Control
Rat brain
Rat brain
其他说明
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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