biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA
Immunoblotting (1:800)
Frozen Sections (1:5000, )
isotype
IgG
shipped in
ambient
Quality Level
Gene Information
human ... DRD2(1813)
rat ... Drd2(24318)
target post-translational modification
unmodified
General description
Anti-Dopamine D₂ Receptor, rabbit polyclonal, specifically recognizes the dopamine D2 receptor in rat brain. Has minimal reactivity with the D2S receptor. It is validated for use in ELISA, WB, & IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa dopamine D2 receptor protein.
Recognizes the dopamine D2 receptor in rat brain. Does not cross-react with other dopamine receptors and exhibits minimal cross-reactivity with the D2S short receptor.
Immunogen
Human
a synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor
Application
ELISA (see comments)
Immunoblotting (1:800)
Frozen Sections (1:5000, see application references)
Immunoblotting (1:800)
Frozen Sections (1:5000, see application references)
Physical form
Undiluted serum.
Preparation Note
Reconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Analysis Note
Positive Control
Rat brain
Rat brain
Other Notes
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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