Eg5 Inhibitor VII - CAS 912953-25-4 - Calbiochem

A cell-permeable quinolinone compound that acts as a highly potent and selective Eg5/KSP inhibitor (K_i = 1.4, 2.1, 2.2, and 1.7 nM for the mutant A133D, KHC-L5, D130V, and the wild-type KSP, respectively) by antagonizing ATP binding via an allosteric site. Effectively induces monopolar spindles in ovarian adenocarcinoma SKOV3 cells and inhibits the proliferation of colorectal carcinoma HCT116 cells (IC₅₀ = 36 and 0.5 nM againt the parental and the ispinesib-resistant D130V mutant line, respectively). It is uncompetitive with respect to microtubule and exhibits little activity against a panel of 7 other mitotic kinesins.

The Eg5 Inhibitor VII controls the biological activity of Eg5. This small molecule/inhibitor is primarily used for Cell Structure applications.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.