Endopeptidase, Neutral, Porcine Kidney

Native, neutral endopeptidase from porcine kidney. A cell surface zinc-containing proteinase that is abundant in the brush border membranes of the kidney proximal tubules. Also present in the brain, endothelial cells, liver, and the lung tissue. Peptidase activity is specifically directed towards the cleavage on the amino side of hydrophobic amino acid residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.

Native, neutral endopeptidase from porcine kidney. A cell surface, zinc containing metallopeptidase that is abundant in the brush border membrane of the kidney proximal tubules. Also present in brain, endothelial cells, liver, and lung. Peptidase activity is specifically directed toward the cleavage on the amino side of hydrophobic residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.

Lyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON^™ X-100 Detergent, pH 7.4.

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.

Barnes, K., et al. 1995. J. Neurochem. 64, 1826.
Gafford, J.T., et al. 1983. Biochemistry 22, 3265.

One unit of enzyme will liberate 1.0 µmol pNA per min from Glutaryl-(Ala)₂-Phe-pNA at 25°C, pH 7.4.

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Toxicity: Standard Handling (A)