PERK, GST-Fusion, Human, Recombinant, E. coli

Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.

Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the α-subunit of eukaryotic translation initiation factor 2 (EIF2α), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening. Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.

Please refer to vial label for lot-specific concentration.

In 50 mM Tris-HCl, 50 mM NaCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

Baltzis, D., et al. 2007. J Biol Chem.43, 31675.
Koumenis, C., et al. 2002. Mol Cell Biol.21, 7405.

Kinase activity is measured as the molar amount of phosphate incorporated into SMAD3 at 30°C using a final concentration of 50 µM ATP.

Specific Activity: Please refer to the lot-specific Certificate of Analysis

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Toxicity: Standard Handling (A)