Epiregulin, Human, Recombinant

Recombinant, human epiregulin consisting of amino acids 63-108 and expressed in E. coli with an N-terminal methionine. Epiregulin, expressed primarily in the placenta and macrophages, stimulates fibroblast, smooth muscle cell, and hepatocyte proliferation.

ED₅₀ = 0.125-0.75 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Mishre, R., et al. 2002. Am. J. Physiol. Renal. Physiol.283, F1151.
Shirakata, Y., et al. 2000. J. Biol. Chem.275, 5748.
Shelly, M., et al. 1998. J. Biol. Chem.273, 10496.
Das, S.K., et al. 1997. Dev. Biol.190, 178.
Komurasaki, T., et al. 1997. Oncogene15, 2841.
Toyoda, H., et al. 1997. Biochem. J.326, 69.
Toyoda, H., et al. 1995. J. Biol. Chem.270, 7495.

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Toxicity: Standard Handling (A)