assay
≥97% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
Quality Level
General description
Recombinant, human fibroblast growth factor 10 (FGF-10) consisting of amino acids 137-208 and expressed in E. Coli. Mature human FGF-10, a ~20 kDa protein, contains a serine-rich region near its N-terminus sharing 93% amino acid sequence identity with mouse FGF-10 and 96% with rat FGF-10.
Biochem/physiol Actions
ED₅₀ = 20-100 ng/ml measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.
Physical form
Lyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Other Notes
Beenken, A. and M. Mohammadi. 2009. Nat. Rev. Drug Discov.8, 235.
Nomura, S., et al. 2008. Br. J. Cancer99, 305.
Zhang, X., et al. 2006. J. Biol. Chem.281, 15694.
Beer, H.D., et al. 2005. Oncogene24, 5269.
Jaskoll, T., et al. 2005. BMC Dev. Biol.5, 11.
Rice, R., et al. 2004. J. Clin. Invest.113, 1692.
Donjacour, A.A., et al. 2003. Dev. Biol.261, 39.
Mailleux, A.A., et al. 2002. Development129, 53.
Sakaue, H., et al. 2002. Genes Dev.16, 908.
Makarenkova, H.P., et al. 2000. Development127, 2563.
Makarenkova, H.P., et al. 2000. Development127, 2563.
Pirvola, U., et al. 2000. J. Neurosci.20, 6125.
Weaver, M., et al. 2000. Development127, 2695.
Igarashi, M., et al. 1998. J. Biol. Chem.273, 13230.
Min, H., et al. 1998. Genes Dev.12, 3156.
Bellusci, S., et al. 1997. Development124, 4867.
Emoto, H., et al. 1997. J. Biol. Chem.272, 23191.
Nomura, S., et al. 2008. Br. J. Cancer99, 305.
Zhang, X., et al. 2006. J. Biol. Chem.281, 15694.
Beer, H.D., et al. 2005. Oncogene24, 5269.
Jaskoll, T., et al. 2005. BMC Dev. Biol.5, 11.
Rice, R., et al. 2004. J. Clin. Invest.113, 1692.
Donjacour, A.A., et al. 2003. Dev. Biol.261, 39.
Mailleux, A.A., et al. 2002. Development129, 53.
Sakaue, H., et al. 2002. Genes Dev.16, 908.
Makarenkova, H.P., et al. 2000. Development127, 2563.
Makarenkova, H.P., et al. 2000. Development127, 2563.
Pirvola, U., et al. 2000. J. Neurosci.20, 6125.
Weaver, M., et al. 2000. Development127, 2695.
Igarashi, M., et al. 1998. J. Biol. Chem.273, 13230.
Min, H., et al. 1998. Genes Dev.12, 3156.
Bellusci, S., et al. 1997. Development124, 4867.
Emoto, H., et al. 1997. J. Biol. Chem.272, 23191.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 3
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