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Merck
CN

371739

Sigma-Aldrich

G-Proteins, Bovine Brain, Functional

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表单

liquid

一般描述

Mixture of purified pertussis toxin-sensitive G-proteins from bovine brain. Suitable for functional studies of G-proteins. Contains the following heterotrimeric G-proteins: Goα (4 - 5 μM), Giα-1 (1 - 2 μM), Giα-2 (1 - 2 μM), and Giα-3 (<1 μM). The βγ-subunit complex associated with these α-subunits represents the predominant form found in bovine brain. Characterized by GTPγS binding activity and by immunoblotting using subunit-specific antisera.

应用

Suitable for GTPγS binding assay and Immunoblotting

制备说明

Following initial thaw, aliquot and freeze (-70°C)

其他说明

Characterized by GTPγS binding and in immunoblot using subunit-specific antisera. Titration is recommended.

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Assay of G-protein beta gamma-subunit complex by catalytic support of ADP-ribosylation of Go alpha.
P J Casey et al.
Methods in enzymology, 195, 315-321 (1991-01-01)
Purification of recombinant Gi alpha and Go alpha proteins from Escherichia coli.
M E Linder et al.
Methods in enzymology, 195, 202-215 (1991-01-01)
P Goldsmith et al.
The Journal of biological chemistry, 262(30), 14683-14688 (1987-10-25)
Antisera AS/6 and 7, raised against a synthetic peptide KENLKDCGLF corresponding to the carboxyl-terminal decapeptide of transducin-alpha, react on immunoblots with purified transducin-alpha and with proteins of 40-41 kDa in all tissues tested. The latter represent one or more forms

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