Anti-Histone H4, Acetylated (2-19), rabbit polyclonal, recognizes the ~10 kDa acetylated histone H4 in Tetrahymena and sodium butyrate-treated HeLa cells. It is validated for use in WB, ICC, and IP.

Protein A purified rabbit polyclonal antibody. Recognizes the ~10 kDa acetylated histone H4 protein.

Recognizes the ~10 kDa acetylated histone H4 protein in Tetrahymena and sodium butyrate-treated HeLa cells.

a tetra-acetylated synthetic peptide [AGG_AcKGG_AcKGMG_AcKVGA_AcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH

Immunoblotting (0.5-2 µg/ml)

Immunocytochemistry (5-10 µg/ml)

Immunoprecipitation (5µg/500 µg lysate)

Please refer to vial label for lot-specific concentration.

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
Tetrahymena and sodium butyrate-treated HeLa cells

Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.

The antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.

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Toxicity: Standard Handling (A)