Hyaluronidase, Ovine Testes

Native hyaluronidase from ovine testes. Catalyzes the hydrolysis of 1,4 linkages between N-acetylhexosamine and D-glucuronate residues in chondroitin, chondroitin-4-sulfate, chrondroitin-6-sulfate esters, and hyaluronic acid. Has an optimal pH of 4.5-6.0. Hydrolyzes the endo-N-acetyl-hexosaminic bonds of hyaluronic acid and chondroitin sulfate A and C to yield tetrasaccharide residues. Hyaluronidase activity is inhibited by the presence of Fe²⁺, Fe³⁺, Cu²⁺, and Mn²⁺.

Note: 1 KU = 1000 units.

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 5 days at 4°C.

Reconstitute at 5 mg/ml in 20 mM sodium phosphate, 0.45% NaCl, and 0.01% BSA, pH 6.9. At a concentration of 5 mg/ml the solution will be clear and colorless.

Lokeshwar, V.B., et al. 2002. J. Biol. Chem.277, 33654.
Borders, C.L., and Raftery, M.A. 1968. J. Biol. Chem. 243, 3756.
DeSalequi, M., et al. 1967. Arch. Biochem. Biophys.121, 548.
Lowry, O.H., et al. 1951. J. Biol. Chem.193, 265.

One unit is defined as the amount of enzyme that will cause the same turbidity reduction as 1.0 unit of International Standard preparation.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)