biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
IPR.1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rabbit, human, canine, mouse, rat, bovine, porcine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)
General description
Purified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Mouse mAb (IPR.1) is validated for use in ELISA, FC, IF, IHC, IP, Radioimmunoassay, Immunoblotting for the detection of IP₃ Receptor.
Immunogen
a synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor
Application
ELISA (1:1000)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
Other Notes
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Specific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable