生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
IPR.1, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
rabbit, human, canine, mouse, rat, bovine, porcine
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG2b
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)
一般描述
Purified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Mouse mAb (IPR.1) is validated for use in ELISA, FC, IF, IHC, IP, Radioimmunoassay, Immunoblotting for the detection of IP₃ Receptor.
免疫原
a synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor
应用
ELISA (1:1000)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
包装
Please refer to vial label for lot-specific concentration.
外形
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
制备说明
Following initial thaw, aliquot and freeze (-70°C).
分析说明
Positive Control
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
其他说明
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Specific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
L Lagostena et al.
The Journal of physiology, 531(Pt 3), 693-706 (2001-03-17)
1. Hensen's cells in the isolated cochlea were stimulated by extracellular adenosine 5'-triphosphate (ATP) applied to their endolymphatic surface while changes in membrane current and intracellular calcium concentration ([Ca2+]i) were measured simultaneously. The response consisted of (i) an initial rapid
John C Hennessey et al.
Pharmacology research & perspectives, 3(2), e00112-e00112 (2015-03-03)
Endothelial cell (EC)-dependent vasodilation by proteinase-activated receptor 2 (PAR2) is preserved in small caliber arteries in disease states where vasodilation by muscarinic receptors is decreased. In this study, we identified and characterized the PAR2-mediated intracellular calcium (Ca(2+))-release mechanisms in EC
L Cavallini et al.
The Journal of biological chemistry, 271(10), 5545-5551 (1996-03-08)
Prostaglandin I2 (PGI2) and sodium nitroprusside (SNP) induce a rapid decay of the thrombin-promoted increase of [Ca2+]i in aspirin-treated platelets incubated in the absence of external Ca2+. The mechanism of their effect was studied with a new method which utilizes
Xudong Feng et al.
Nature communications, 5, 4487-4487 (2014-07-30)
Inositol 1, 4, 5-trisphosphate receptor (IP3R)-mediated Ca(2+) release from the endoplasmic reticulum (ER) triggers many physiological responses in neurons, and when uncontrolled can cause ER stress that contributes to neurological disease. Here we show that the unfolded protein response (UPR)
Chenxi Guo et al.
Journal of cell science, 133(13) (2020-06-18)
The role of two-pore channel type 2 (TPC2, encoded by tcpn2)-mediated Ca2+ release was recently characterized in zebrafish during establishment of the early spinal circuitry, one of the key events in the coordination of neuromuscular activity. Here, we extend our
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