产品名称
Anti-IP3 Receptor Mouse mAb (IPR.1), liquid, clone IPR.1, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
IPR.1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rabbit, human, canine, mouse, rat, bovine, porcine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)
Analysis Note
Positive Control
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
Application
ELISA (1:1000)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Mouse mAb (IPR.1) is validated for use in ELISA, FC, IF, IHC, IP, Radioimmunoassay, Immunoblotting for the detection of IP₃ Receptor.
Immunogen
a synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor
Other Notes
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Specific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
L Lagostena et al.
The Journal of physiology, 531(Pt 3), 693-706 (2001-03-17)
1. Hensen's cells in the isolated cochlea were stimulated by extracellular adenosine 5'-triphosphate (ATP) applied to their endolymphatic surface while changes in membrane current and intracellular calcium concentration ([Ca2+]i) were measured simultaneously. The response consisted of (i) an initial rapid
John C Hennessey et al.
Pharmacology research & perspectives, 3(2), e00112-e00112 (2015-03-03)
Endothelial cell (EC)-dependent vasodilation by proteinase-activated receptor 2 (PAR2) is preserved in small caliber arteries in disease states where vasodilation by muscarinic receptors is decreased. In this study, we identified and characterized the PAR2-mediated intracellular calcium (Ca(2+))-release mechanisms in EC
L Cavallini et al.
The Journal of biological chemistry, 271(10), 5545-5551 (1996-03-08)
Prostaglandin I2 (PGI2) and sodium nitroprusside (SNP) induce a rapid decay of the thrombin-promoted increase of [Ca2+]i in aspirin-treated platelets incubated in the absence of external Ca2+. The mechanism of their effect was studied with a new method which utilizes
Xudong Feng et al.
Nature communications, 5, 4487-4487 (2014-07-30)
Inositol 1, 4, 5-trisphosphate receptor (IP3R)-mediated Ca(2+) release from the endoplasmic reticulum (ER) triggers many physiological responses in neurons, and when uncontrolled can cause ER stress that contributes to neurological disease. Here we show that the unfolded protein response (UPR)
Chenxi Guo et al.
Journal of cell science, 133(13) (2020-06-18)
The role of two-pore channel type 2 (TPC2, encoded by tcpn2)-mediated Ca2+ release was recently characterized in zebrafish during establishment of the early spinal circuitry, one of the key events in the coordination of neuromuscular activity. Here, we extend our
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