Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli

Full-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.

ED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.

Sterile-filtered prior to lyophilization. Contains no additives.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.

Reconstitute with sterile H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffer before use.

Clemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res.14, 7873.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)