Interleukin-2, Human, Recombinant, E. coli

Recombinant, human interleukin-2 expressed in E. coli. Has a variety of immunological functions. Acts as an autocrine agent to promote proliferation and maturation of activated T cells. A comparison of the amino acid sequences of human and murine IL-2 shows ~60% sequence similarity. Inhibits dexamethasone-induced apoptosis in T-lymphocytes. Induces nitric oxide synthase. Human IL-2 is active in murine cell lines. Validated for use in cell culture and bioassays.

ED₅₀= 80-500 pg/ml as measured in a cell proliferation assay with an IL-2-dependent murine cytotoxic T cell line

Carrier-free

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration of 0.1-1 mg/ml with sterile 100 mM acetic acid. Further dilute in buffer or medium containing ≥0.1% BSA.

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Maeda, A., et al. 1983. Biochem. Biophys. Res. Commun.115, 1040.
Taniguchi, T., et al. 1983. Nature302, 305.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)