Interleukin-8, Human, Recombinant, E. coli

Recombinant, human interleukin-8 expressed in E. coli. A potent proinflammatory cytokine produced by monocytes, fibroblasts, and keratinocytes in response to stimulation by LPS, IL-1, or TNF-α, and by T lymphocytes in response to PHA stimulation. Induces adhesion of neutrophils to endothelial cells. A physiological initiator of chemotactic migration of leukocytes. May be involved in chronic inflammation. IL-8 acts as an angiogenic factor in human gastric carcinoma.

ED₅₀ = 150-300 ng/ml as measured by induction of myeloperoxidase release from human neutrophils

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Kido, S., et al. 2001. Eur. J. Cancer37, 1482.
Wilson, A.J., et al. 1999. Clin. Sci. 97, 385.
Harada, A., et al. 1994. J. Leukoc. Biol.56, 559.
Mazzucchelli, L., et al. 1994. Am. J. Pathol. 144, 997.
Oppenheim, J.J., et al. 1991. Annu. Rev. Immunol.9, 617.
Matsushima, K., et al. 1988. J. Exp. Med.167, 1883.
Schröeder, J., et al. 1987. J. Immunol.139, 3474.

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Toxicity: Standard Handling (A)