assay
≥95% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Native, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydrolases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity. Recommended reaction buffer: 25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 minutes with 10 mM DTT to achieve maximum activity.
Biochem/physiol Actions
Fully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075)
Physical form
In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Dang, L.C., et al. 1998. Biochemistry 37, 1868.
Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
Stein, R.L., et al. 1995. Biochemistry 34, 12616.
Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.
Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
Stein, R.L., et al. 1995. Biochemistry 34, 12616.
Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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