biological source
mouse
antibody product type
primary antibodies
clone
T218, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgM
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Mouse monoclonal antibody. Recognizes the Lewis b carbohydrate chain.
Recognizes the Lewis b carbohydrate chain in colon adenocarcinoma.
This Anti-Lewis b Mouse mAb (T218) is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Lewis b.
Immunogen
Human
human SK-CO-10 colon cancer cells
Application
ELISA (see comments)
Immunoblotting (see comments)
Immunohistochemistry (1:10-1:40)
Immunoblotting (see comments)
Immunohistochemistry (1:10-1:40)
Physical form
In PBS, 1% BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Colon adenocarcinoma tissue
Colon adenocarcinoma tissue
Other Notes
Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
For immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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