生物来源
mouse
质量水平
抗体产品类型
primary antibodies
克隆
T218, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgM
运输
wet ice
储存温度
−20°C
一般描述
Mouse monoclonal antibody. Recognizes the Lewis b carbohydrate chain.
Recognizes the Lewis b carbohydrate chain in colon adenocarcinoma.
This Anti-Lewis b Mouse mAb (T218) is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Lewis b.
免疫原
Human
human SK-CO-10 colon cancer cells
应用
ELISA (see comments)
Immunoblotting (see comments)
Immunohistochemistry (1:10-1:40)
Immunoblotting (see comments)
Immunohistochemistry (1:10-1:40)
外形
In PBS, 1% BSA.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Colon adenocarcinoma tissue
Colon adenocarcinoma tissue
其他说明
Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
For immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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