Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% Brij^® 35 detergent, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.

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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)