assay
≥95% (HPLC)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
Quality Level
General description
Native 2.5S nerve growth factor from mouse submaxillary glands. Enhances the survival, phagocytosis, and generation of superoxide in murine neutrophils. Reported to stimulate extracellular matrix invasion by human myeloma cells.
Biochem/physiol Actions
ED₅₀ ≤ 15 ng/ml using a PC-12 neurite outgrowth assay
Physical form
Lyophilized from sterile-filtered PBS.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 weeks at -20°C.
Reconstitute to ≥10 µg/ml in sterile H₂O or buffer containing 2% BSA.
Other Notes
Hermann, J.L., et al. 1993. Mol. Cell. Biol. 4, 1205.
Raffioni, S., and Bradshaw, R.A. 1992. Proc. Natl. Acad. Sci. USA89, 9121.
Traverse, S., et al. 1992. Biochem. J.288, 351.
Levi-Montalcini, R. 1987. In Vitro Cell. Dev. Biol.23, 227.
Raffioni, S., and Bradshaw, R.A. 1992. Proc. Natl. Acad. Sci. USA89, 9121.
Traverse, S., et al. 1992. Biochem. J.288, 351.
Levi-Montalcini, R. 1987. In Vitro Cell. Dev. Biol.23, 227.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1
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