生物来源
rabbit
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA (1:1000)
Immunoblotting
Immunoelectrophoresis
Immunoprecipitation )
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ELANE(1991)
一般描述
Ammonium sulfate precipitated rabbit polyclonal antibody. Recognizes human neutrophil elastase.
Recognizes the neutrophil elastase protein.
This Anti-Neutrophil Elastase Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoelectrophoresis, Immunoprecipitation for the detection of Neutrophil Elastase.
免疫原
Human
human neutrophil elastase
应用
ELISA (≥ 1:1000)
Immunoblotting (see comments)
Immunoelectrophoresis (see comments)
Immunoprecipitation (see comments)
Immunoblotting (see comments)
Immunoelectrophoresis (see comments)
Immunoprecipitation (see comments)
包装
Please refer to vial label for lot-specific concentration.
外形
In PBS, pH 7.5.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
其他说明
Single arc by immunoelectrophoresis against neutrophil granule extract and human neutrophil elastase. This antibody has also been reported to work for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Avinash Khandagale et al.
Journal of leukocyte biology, 104(6), 1199-1213 (2018-08-15)
Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial
Ava Hosseinzadeh et al.
Frontiers in immunology, 3, 391-391 (2012-12-28)
To prevent the spread of pathogens neutrophils as the first line of defense are able to release Neutrophil Extracellular Traps (NETs), a recently discovered form of immune response. Reactive oxygen species (ROS) have been shown to be essential for many
Volker Brinkmann et al.
Frontiers in immunology, 3, 413-413 (2013-01-15)
Neutrophil Extracellular Traps (NETs) consist of decondensed chromatin studded with granular and some cytoplasmic proteins. They are formed by activated neutrophil granulocytes, also called polymorphonuclear leukocytes (PMN) as the result of an active cell death program, named NETosis. NETosis can
Transparent reporting of all GRADE criteria in clinical practice guidelines.
Nicole Boluyt et al.
American journal of respiratory and critical care medicine, 185(10), 1129-1129 (2012-05-17)
Nancy McCartney-Francis et al.
Journal of leukocyte biology, 96(5), 917-929 (2014-07-18)
SLPI, a potent epithelial and myeloid-derived serine protease inhibitor with antimicrobial and anti-inflammatory functions, is induced by the intracellular parasite Leishmania major, and increased SLPI expression is evident within lesions that follow L. major infection. In contrast to self-resolving infection
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