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Merck
CN

481413

Aurora A Kinase, His•Tag®, Human, Recombinant, S. frugiperda

Aurora A Kinase is a full-length, recombinant (2-403) form fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda using a baculovirus expression system.

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UNSPSC Code:
12352202
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Quality Level

assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥120 unit/μg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Full-length, recombinant, human Aurora A kinase (amino acids 2-403) (GenBank Accession: NM_003600) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Aurora A plays an important role in chromosome segregation and cell division.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 Detergent, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
Ouchi, M., et al. 2004. J. Biol. Chem.279, 19643.
Kunitoku, N., et al. 2003. Dev. Cell5, 853.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Toxicity: Regulatory Review (Z)


法规信息

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