484400
头孢硝噻
≥95% (UV), solid, β-lactamase substrate, Calbiochem®
别名:
头孢硝噻, 3-（2，4-二硝基苯乙烯基）-（6R，7R）-7-（2-噻吩基乙酰胺基）-头孢-3-EM-4-羧酸，E-异构体
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关于此项目
产品名称
头孢硝噻, A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase.
SMILES string
[s]1c(ccc1)CC(=O)N[C@H]2[C@H]3SCC(=C(N3C2=O)C(=O)O)\C=C\c4c(cc(cc4)[N+](=O)[O-])[N+](=O)[O-]
InChI
1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1
InChI key
LHNIIDJCEODSHA-OQRUQETBSA-N
assay
≥95% (UV)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
orange-yellow
solubility
soluble (Preparing a Nitrocefin (500 µg/ml) Solution
• Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex.
• Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume.
• This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications.
• Nitrocefin, particularly in solution, is very sensitive to light.)
shipped in
ambient
storage temp.
−20°C
Quality Level
相关类别
Disclaimer
General description
Other Notes
King, A., et al. 1980.Antimicrob.Agents Chemother.17, 165.
Matthew, M., et al. 1975.J. Gen.Microbiol.88, 169.
O’Callaghan, C.H., et al. 1972.Antimicrob.Agents Chemother.1, 283.
Packaging
Preparation Note
•将1 mg头孢硝噻吩溶于100 µl二甲亚砜（DMSO）中并涡旋。
• 加入1.9 ml磷酸盐缓冲液（100 mM，pH值7），以产生2 ml总体积。
•这样就产生了500 µg/ml（〜1 mM）的头孢硝噻吩工作溶液，适用于大多数应用。
•头孢硝噻吩对光非常敏感， 特别是在溶液中时。
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
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