iPSC Induction Enhancer II, OAC1

A cell-permeable pyrrolopyridinyl-benzamide compound that is reported to enhance the efficiency of 4F/4TF- (OSKM; Oct44, Sox2, Klf4, c-Myc) driven iPSC generation from MEFs cultured in either feeder cell-containing ESC media (induction efficiency 20 d post viral transduction = 0.43% vs. 0.186%, respectively, with or without 1 µM OAC1 treatment from d2 to d20) or in feeder-/serum-free iSF1 media (efficiency 10 d post viral transduction = 2.75% vs. 0.68%, respectively, with or without 1 µM OAC1 treatment from d2 to d10). OCA1 is shown to increase cellular Oct4, Nanog, Sox2, and Tet1 mRNA levels via a yet unidentified mechanism, although OAC1 is reported not to affect Oct4 promoter methylation, cellular Wnt signaling, or p53 and p21 mRNA levels. Six other iPSC reprogramming improving reagents, AM580, AzaC, BIX-01294 (Cat. No. 382190), Tranylcypromine (Cat. No. 616431), VPA (Cat. No. 676380), and Vitamin C (Cat. No. 1813), in comparison, do not exhibit Oct4 and Nanog promoters enhancing activity, while BIO (Cat. No. 361550) is demonstrated to be 3.8- and 6-fold less potent than OAC1, respectively, in inducing Oct4 and Nanog transcription (in human ESCs-based reporter assays).

Cell permeable: yes

Reversible: yes

Packaged under inert gas

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Use only fresh DMSO for reconstitution.

Li, W., et al. 2012. Proc. Natl. Acad. Sci. USA109, 20853.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)