DNA Methyltransferase Inhibitor V, RSC133

A cell-permeable compound that targets Dnmt1 at the cofactor SAM-binding site and enhances the efficiency of iPSC production from both murine MEF and human foreskin fibroblasts.

A cell-permeable indolylacrylamido-benzamide compound that is reported to target Dnmt1 at the cofactor SAM-binding site and enhance the efficiency of iPSC production from both murine MEF (10 µM starting 5 d post KSOM viral infection) and human foreskin fibroblast (10 µM starting same day as or 5 d post KSOM viral infection) cultures with accompanying cellular Dnmt1 activity reduction, affecting hFF viability only at high concentrations (78% of control post 72 h 100 µM treatment). Also shown to help maintain H9 hESCs in undifferentiated state when cultured in unconditioned media (10 µM).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Cell permeable: yes

Primary Target
Dnmt1

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Use only fresh DMSO for reconstitution.

Lee, J., et al. 2012. Angew. Chem. Int. Engl.51, 12509.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)