Autophagy Inhibitor IV, Cpd18

A cell-permeable 3-methyladenine (3-MA) derivative that acts as an autophagy inhibitor via the inhibition of class III PI3K, and without touching class I PI3K. Shown to block 80% of starvation-induced autophagy at 1 mM (IC₅₀ = 0.67 mM, compared with 3-MA at 6 mM). Displays much better solubility than 3-MA (5 times more soluble in water and 30 times more soluble in DMSO). Also found to reduce cell viability by 55% at 1 mM at 12 hours compared with 72% reduction for 3-MA at 10 mM.

Please note that the molecular weight for this compound is lot-specific due to variable water content.

Primary Target
PI3K

Reversible: yes

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Wu, Y., et al, 2013. Autophagy9, 595.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)