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Merck
CN

506165

p53, His•Tag®, Human, Recombinant, E. coli

别名:

Tumor Protein p53

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UNSPSC Code:
12352203

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assay

≥80% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

General description

This product has been discontinued.



Recombinant, human, wild-type p53 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.

Recombinant, human, wild-type p53 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.

Biochem/physiol Actions

Typical DNA binding assays require 0.5 ng to 2 ng of p53 protein per assay.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM KCl, 30 mM sodium pyrophosphate, 5% glycerol, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Liu, G., et al. 2003. J. Biol. Chem.278, 17557.
Zhang, L., et al. 2000. Cancer Res.60, 3655.
Fields, S., and Jang, S.K. 1990. Science249, 1046.
Michalovitz, D., et al. 1990. Cell62, 671.
Raycroft, L., et al. 1990. Science249, 1049.
Finlay, C.A., et al. 1989. Cell57, 1083.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

存储类别

10-13 - German Storage Class 10 to 13

法规信息

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