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Merck
CN

506165

Sigma-Aldrich

p53, His•Tag®, Human, Recombinant, E. coli

别名:

Tumor Protein p53

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UNSPSC代码:
12352203

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方案

≥80% (SDS-PAGE)

质量水平

100

表单

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

异质活性

Proteases, none detected

运输

wet ice

储存温度

−70°C

一般描述

This product has been discontinued.



Recombinant, human, wild-type p53 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.

Recombinant, human, wild-type p53 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.

生化/生理作用

Typical DNA binding assays require 0.5 ng to 2 ng of p53 protein per assay.

包装

Please refer to vial label for lot-specific concentration.

外形

In 50 mM KCl, 30 mM sodium pyrophosphate, 5% glycerol, pH 7.5.

制备说明

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Liu, G., et al. 2003. J. Biol. Chem.278, 17557.
Zhang, L., et al. 2000. Cancer Res.60, 3655.
Fields, S., and Jang, S.K. 1990. Science249, 1046.
Michalovitz, D., et al. 1990. Cell62, 671.
Raycroft, L., et al. 1990. Science249, 1049.
Finlay, C.A., et al. 1989. Cell57, 1083.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Toxicity: Standard Handling (A)

储存分类代码

10-13 - German Storage Class 10 to 13

法规信息

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