assay
≥99% (HPLC)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles, desiccated (hygroscopic), protect from light
storage temp.
−70°C
Quality Level
General description
Active hormonal form of vitamin D, formed by the renal 1-hydroxylation of 25-(OH)D3. Has immunomodulatory properties in vitro and in vivo. Prevents the development of clinical diabetes in NOD mice, which are an animal model of human autoimmune diabetes. Induces reversible hydrolysis of the sphingomyelin cycle and generates ceramide. Stimulates intestinal Ca2+ and phosphate transport and mobilizes Ca2+ from bone. Involved in cellular differentiation, carcinogenesis, and insulin secretion.
Application
- Evaluation system for cell-permeable CYP3A4 inhibitory activity using 1α,25-dihydroxy-vitamin D(3)-induced intestinal cell lines.: Ueno et al. developed an evaluation system for cell-permeable CYP3A4 inhibitory activity utilizing 1α,25-dihydroxy-vitamin D3-induced intestinal cell lines. This system helps in screening for CYP3A4 inhibitors, which are critical in drug metabolism and interaction studies (Ueno et al., 2021).
Biochem/physiol Actions
Primary Target
vitamin D receptor
vitamin D receptor
Packaging
Packaged under inert gas
Physical form
A 1 mM (50 ug/112 µL) solution of Vitamin D3, 1α, 25-Dihydroxy- (Cat. No. 679101) in DMSO.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
Other Notes
Semizarov, D., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15412.
Hannun, Y.A. 1994. J. Biol. Chem.269, 3125.
Mathieu, C., et al. 1994. Diabetologica37, 552.
Thomasset, M. 1994. Pathol. Biol.42, 163.
Horton, W.E., Jr., et al. 1991. J. Biol. Chem.266, 24804.
Larsen, C.G., et al. 1991. Biochem. Biophys. Res. Commun.176, 1020.
Thavarajah, M., et al. 1991. Biochem. Biophys. Res. Commun.176, 1189.
Wakasugi, M., et al. 1991. Prostaglandins42, 127.
Matsumoto, K., et al. 1990. Biochem. Biophys. Res. Commun.166, 916.
Simpson, R.U., et al. 1989. J. Biol. Chem.264, 19710.
DeLuca, H.F., and Schnoes, H.K. 1983. Annu. Rev. Biochem.52, 411.
Hannun, Y.A. 1994. J. Biol. Chem.269, 3125.
Mathieu, C., et al. 1994. Diabetologica37, 552.
Thomasset, M. 1994. Pathol. Biol.42, 163.
Horton, W.E., Jr., et al. 1991. J. Biol. Chem.266, 24804.
Larsen, C.G., et al. 1991. Biochem. Biophys. Res. Commun.176, 1020.
Thavarajah, M., et al. 1991. Biochem. Biophys. Res. Commun.176, 1189.
Wakasugi, M., et al. 1991. Prostaglandins42, 127.
Matsumoto, K., et al. 1990. Biochem. Biophys. Res. Commun.166, 916.
Simpson, R.U., et al. 1989. J. Biol. Chem.264, 19710.
DeLuca, H.F., and Schnoes, H.K. 1983. Annu. Rev. Biochem.52, 411.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
188.6 °F - (refers to pure substance)
flash_point_c
87 °C - (refers to pure substance)
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