assay
≥90% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
Quality Level
General description
Recombinant, mouse pentraxin 3, consisting of amino acids 18-381 and expressed in NS0-derived murine myeloma cells with a C-terminal His•Tag. Pentraxin 3, a secreted glycoprotein, exhibits a C-terminal Pentraxin domain and a unique N-terminal domain. Its increased expression may enhance the immune response to protect a host from infection.
Biochem/physiol Actions
Functional ELISA: binds rhC1q (5 µg/ml) in the linear range of 0.25-15 µg/ml.
Physical form
Lyophilized from 0.2 µm filtered MOPS, NaCl, CaCl₂, BSA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Other Notes
Dias, A.A.M. et al. 2001. J. Leukocyte Biol.69, 928.
Luchetti, M.M. et al. 2000. Clin. Exp. Immunol.119, 196.
Rovere, P. et al. 2000. Blood96, 4300.
Bottazzi, B. et al. 1997. JBC. 272, 32817.
Goodman A.R. et al. 1996. Cytokine & Growth Factor Rev.7, 191.
Introna, M. et al. 1996. Blood87, 1862.
Altmeyer, A. et al. 1995. J. Biol. Chem.270, 25584.
Lee, G.W. et al. 1993. J. Immunol.150, 1804.
Breviario, F. et al. 1992. JBC.267, 22190.
Lee, T.H. et al. 1990. Mol. Cell. Biol.10, 1982.
Osmand, A.P. et al. 1977. PNAS, USA74, 739.
Luchetti, M.M. et al. 2000. Clin. Exp. Immunol.119, 196.
Rovere, P. et al. 2000. Blood96, 4300.
Bottazzi, B. et al. 1997. JBC. 272, 32817.
Goodman A.R. et al. 1996. Cytokine & Growth Factor Rev.7, 191.
Introna, M. et al. 1996. Blood87, 1862.
Altmeyer, A. et al. 1995. J. Biol. Chem.270, 25584.
Lee, G.W. et al. 1993. J. Immunol.150, 1804.
Breviario, F. et al. 1992. JBC.267, 22190.
Lee, T.H. et al. 1990. Mol. Cell. Biol.10, 1982.
Osmand, A.P. et al. 1977. PNAS, USA74, 739.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 3
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