Pertussis Toxin, A Protomer, Bordetella pertussis - Calbiochem

Enzymatic component of the holotoxin. Has NAD-glycohydrolase activities. Unable to penetrate cells in the absence of the B oligomer.

Cell permeable: no

Primary Target
Has both NAD-glycohydrolase and ADP-ribosyltransferase activities

Product does not compete with ATP.

Reversible: no

Lyophilized from 10 mM Tris, 100 µM EDTA, 0.04% CHAPS, pH 8.0.

Following reconstitution, refrigerate (4°C). Stock solutions, prepared from high ionic strength buffers, are stable for up to 3 months at 4°C.

Reconstitute with 500 µl sterile dH₂O or high ionic buffer.

Single major band by SDS-PAGE

Brito, G.A., et al. 1997. Infect. Immun. 65, 1114.
Gill, D.M., and Coburn, J., 1988. Biochim. Biophys. Acta954, 65.
Kaslow, H.R., and Lesikar, D.D., 1987. Biochemistry26, 4397.
Burns, D.L., and Manclark, C.R., 1986. J. Biol. Chem.261, 4324.
Moss, J., et al. 1983. J. Biol. Chem.258, 11879.
Tamura, M., et al. 1982. Biochemistry21, 5516.

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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Toxic (F)