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Merck
CN

521215

PDGF, AA Homodimer, Human, Recombinant, E. coli

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UNSPSC Code:
12352202
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assay

≥97% (SDS-PAGE)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1 EU/μg Endotoxin (EU/μg PDGF-AA)

shipped in

ambient

storage temp.

−70°C

Quality Level

General description

Recombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that deduced from the native human nucleotide sequence except for the addition of an N-terminal methionine group.

Biochem/physiol Actions

1-3 ng/ml

Physical form

Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.

Preparation Note

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
Reconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.

Other Notes

Butt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res.51, 5087.
Betsholtz, C., et al. 1986. Nature320, 695.
Raines, E.W., et al. 1985. Methods Enzymol.109, 749.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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