A cell permeable adenine analog that is widely used as a versatile fluorescent probe to investigate RNA and DNA secondary and tertiary structures and their conformation dynamics in response to local environment when interacting with other biomolecules with great sensitivity. Forms stable base pairs with neucleobase uracil found in RNA and thymine in DNA, and moderately stable base pairs with cytosine. Exhibits intensive fluorescence emission (lAbsmax = 303 nM, lflmax = 370 nM, and fluorescence quantum yield in solution QFI = 0.68) and longer excitation wavelength compared with other neucleobases enabling its selective excitation with low background signals.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Cell permeable: yes

Primary Target
DNA and RNA

Reversible: yes

Packaged under inert gas

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Ma, L., et al. 2014. Biochem. Biophys. Res. Comm.449, 120.
Widom, J. R., et al. 2013. New. J. Phys.15, 025028.
Souliere, M. F., et al. 2011. J. Am. Chem. Soc.133, 16161,
Jean, J. M., et al. 2001. PNAS98,37.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Harmful (C)