assay
≥90% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, mouse progranulin, consisting of amino acids 18-589, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. Progranulin is an ubiquitously expressed, secreted glycoprotein that is involved in regulation of cell proliferation.
Biochem/physiol Actions
Significantly enhances neurite outgrowth of E18 rat embryonic cortical neurons using 4-16 µg/ml progranulin immobilized on nitrocelluose-coated microplates.
Physical form
Lyophilized from 0.2 µm filtered PBS.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 250 µg/ml with sterile PBS.
Other Notes
Liu, Y. et al. 2007. BMC Cancer7, 22.
Xu, K. et al. 2007. J. Biol. Chem.282, 11347.
Barreda, D.R. et al. 2004. Dev. Comp. Immunol.28, 727.
Davidson, B. et al. 2004. Cancer100, 2139.
Sun, X. et al. 2004. Am. J. Respir. Cell Mol. Biol.30, 510.
Gonzalez, E.M. et al. 2003. J. Biol. Chem.278, 38113.
He, Z. et al. 2003. Nat. Med.9, 225.
He, Z. and Bateman, A. 2003. J. Mol. Med.81, 600.
Jones, M.B. et al. 2003. Gynecol. Oncol.88, S136.
Wang, W. et al. 2003. Clin. Cancer Res.9, 2221.
Suzuki, M. and Nishiahara, M. 2002. Mol. Genet. Metab.75, 31.
Zhu, J. et al. 2002. Cell111, 867.
Lu, R. and Serrero, G. 2000. Proc. Natl. Acad. Sci. U.S.A.97, 3993.
Justen, H.P. et al. 2000. Mol. Cell Biol. Res. Commun.3, 165.
Zanocco Marani, T. et al. 1999. Cancer Res.59, 5331.
Zhang, H. and G. Serrero 1998. Proc. Natl. Acad. Sci. U.S.A.95, 14202.
Zhou, J. et al. 1993. J. Biol. Chem.268, 10863.
Plowman, G.D. et al. 1992. J. Biol. Chem.267, 13073.
Bateman, A. et al. 1990. Biochem. Biophys. Res. Commun.173, 1161.
Xu, K. et al. 2007. J. Biol. Chem.282, 11347.
Barreda, D.R. et al. 2004. Dev. Comp. Immunol.28, 727.
Davidson, B. et al. 2004. Cancer100, 2139.
Sun, X. et al. 2004. Am. J. Respir. Cell Mol. Biol.30, 510.
Gonzalez, E.M. et al. 2003. J. Biol. Chem.278, 38113.
He, Z. et al. 2003. Nat. Med.9, 225.
He, Z. and Bateman, A. 2003. J. Mol. Med.81, 600.
Jones, M.B. et al. 2003. Gynecol. Oncol.88, S136.
Wang, W. et al. 2003. Clin. Cancer Res.9, 2221.
Suzuki, M. and Nishiahara, M. 2002. Mol. Genet. Metab.75, 31.
Zhu, J. et al. 2002. Cell111, 867.
Lu, R. and Serrero, G. 2000. Proc. Natl. Acad. Sci. U.S.A.97, 3993.
Justen, H.P. et al. 2000. Mol. Cell Biol. Res. Commun.3, 165.
Zanocco Marani, T. et al. 1999. Cancer Res.59, 5331.
Zhang, H. and G. Serrero 1998. Proc. Natl. Acad. Sci. U.S.A.95, 14202.
Zhou, J. et al. 1993. J. Biol. Chem.268, 10863.
Plowman, G.D. et al. 1992. J. Biol. Chem.267, 13073.
Bateman, A. et al. 1990. Biochem. Biophys. Res. Commun.173, 1161.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1