NCK/PAK Blocking Peptide

Cell permeable: yes

Primary Target
NCK

Reversible: yes

Toxicity: Standard Handling (A)

A cell-permeable synthetic peptide derived from the proline-rich region near the N-terminal of p21 activated kinase1 (PAK1) and fused with HIV TAT. Shown to selectively binds to the non-catalytic region of tyrosine kinase adaptor protein (NCK) and disrupts the cellular transport and localization of PAK. Shown to specifically inhibit endothelial cell migration and contractility (IC₅₀ = 2 µM) and block bFGF-induced angiogenesis in a chick chorioallantoic membrane (CAM) assay. Does not exhibit any deleterious effects on endothelial cell survival and growth.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Chen, J., et al. 2015, Sci. Signal.8, 365.

Kiosses, W., et al. 2002, Circ. Res.90, 697.

H2N-YGRKKRRQRRRGKPPAPPMRNTSTM-C02H

Packaged under inert gas

Supplied as TFA salt.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany