Native, rabbit 20S proteasome. Catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP-dependent process. Can be activated by PA28 (Cat. No. 506280) or SDS.

Fully functional as measured using Z-LLVY-AMC as the substrate following SDS activation

Please refer to vial label for lot-specific concentration.

In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.

Following initial thaw, aliquot and freeze (-70°C).

Stein, R.L., et al. 1996. Biochemistry 35, 3899.
Ciechanover, A. 1994. Cell 79, 13.
Dick, L.R., et al. 1994. J. Immunol. 152, 3884.
Dick, L.R., et al. 1991. Biochemistry 30, 2725.
Hough, R., et al. 1987. J. Biol. Chem. 262, 8303.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)