539481-M
PKA, Catalytic Subunit, Mouse, Recombinant, E. coli
别名:
cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
form
liquid
specific activity
≥2.5 x 106 U/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
foreign activity
Phosphatases, proteases, none detected
storage temp.
−20°C
Quality Level
General description
Recombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP3-induced Ca2+ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Grant, B.D., and Adams, J.A. 1996. Biochemistry35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem.271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci.13, 5242.
Brindle, P., et al. 1993. Nature364, 821.
Hajnoczky, G., et al. 1993. Biochem. J.293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10135.
Wang, L-Y., et al. 1993. Science259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem.264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem.243, 3763.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem.271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci.13, 5242.
Brindle, P., et al. 1993. Nature364, 821.
Hajnoczky, G., et al. 1993. Biochem. J.293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10135.
Wang, L-Y., et al. 1993. Science259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem.264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem.243, 3763.
One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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