Recombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP₃-induced Ca²⁺ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.

Following initial thaw, aliquot and freeze (-20°C).

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