assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥8,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
color
clear colorless
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Recombinant, human PP2Cα fused to a His•Tag sequence and expressed in E. coli. A Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response, gene expression and replication in eukaryotes. Recently it has been shown that PP2Cα inhibits cell growth and activates the p53 pathway.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Ofek, P., et al. 2003. J. Biol. Chem.278, 14299.
Das, A.K., et al. 1996. EMBO J.15, 6798.
Das, A.K., et al. 1996. EMBO J.15, 6798.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 37°C, pH 7.4 using 10 mM of substrate.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Thuy-Ai Nguyen et al.
DNA repair, 9(7), 813-823 (2010-05-11)
Nucleotide excision repair (NER) is the only mechanism in humans to repair UV-induced DNA lesions such as pyrimidine (6-4) pyrimidone photoproducts and cyclobutane pyrimidine dimers (CPDs). In response to UV damage, the ataxia telangiectasia mutated and Rad3-related (ATR) kinase phosphorylates
George G Schweitzer et al.
Journal of applied physiology (Bethesda, Md. : 1985), 113(12), 1852-1861 (2012-09-01)
Prior exercise by rats can induce a sustained increase in muscle Akt substrate of 160 kDa (AS160) phosphorylation on Thr(642) (pAS160(Thr642)). Because phosphorylation of AS160 on both AS160(Thr642) and AS160(Ser588) is important for insulin-stimulated glucose transport (GT), we determined if
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