PPI2, Human, Recombinant, E. coli

Recombinant, human PPI2 expressed in E. coli. Inhibitor-2 is the heat-stable, regulatory subunit of the cytosolic type 1 Ser/Thr protein phosphatase-1 (PP1). Inhibits the activity of the free catalytic subunit of PP1 (IC₅₀ = 1 nM). Inhibition is reported to involve a "masking region" on the inhibitor that lies in close proximity to the active site of PP1 and blocks the access to substrates. Interaction of inhibitor-2 with the catalytic subunit of PP1 leads first to a rapid inhibition of the enzyme activity followed by the formation of a stable inactive complex. The inactive complex can be reactivated through phosphorylation of inhibitor-2 at Thr⁷² by glycogen synthase kinase-3 (GSK-3).

Cell permeable: no

Primary Target
activity of free catalytic subunit of PP1

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 1 nM inhibiting the activity of the free catalytic subunit of PP1

Following reconstitution, aliquot and freeze (-20°C).

Reconstitute with 50 mM Tris-HCl, pH 7.0.

Yang, J., et al. 2000. J. Biol. Chem.275, 22635.
Huang, H.B., et al. 1999. J. Biol. Chem.274, 7870.
Helps, N.R., et al. 1994. FEBS Lett.340, 93.
Park, I., et al. 1994. J. Biol. Chem.269, 944.
Cohen, P. 1991. Methods Enzymol.201, 389.

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Toxicity: Standard Handling (A)