assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥800 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Full-length, recombinant, human PKCβI expressed in S. frugiperda insect cells. Suitable for studies of PKCβI and its regulation. PKCβI is a Ca2+ and phospholipid-dependent enzyme.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON™ X-100 Detergent, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Newton, A.C. 1995. J. Biol. Chem.270, 28495.
Dekker, L.V. and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M. and Burns, D.J. 1991. J. Biol. Chem.266, 4661.
Dekker, L.V. and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M. and Burns, D.J. 1991. J. Biol. Chem.266, 4661.
One unit is defined as the amount of enzyme that will transfer 1.0 nmol of phosphate to histone H1 per min at 30°C, pH 7.4.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Disclaimer
Toxicity: Standard Handling (A)
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