form
liquid
assay
≥95% (SDS-PAGE)
specific activity
≥50,000 U/mg, ≥50,000 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
foreign activity
Proteases, serine/threonine phosphatases, none detected
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
This product has been discontinued.
Recombinant, 51 kDa catalytic domain of the Yersinia protein tyrosine phosphatase containing a C235R mutation and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.
Physical form
In 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% Brij® 35 Detergent, 50% glycerol, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.2.
Zhang, Z.Y., et al. 1992. J. Biol. Chem.267, 23759.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Guan, K. and Dixon, J.E. 1990. Science249, 653.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Guan, K. and Dixon, J.E. 1990. Science249, 653.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10-13 - German Storage Class 10 to 13
法规信息
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