方案
≥95% (SDS-PAGE)
质量水平
表单
liquid
比活
≥50,000 U/mg
≥50,000 units/mL
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
异质活性
Proteases, serine/threonine phosphatases, none detected
运输
wet ice
储存温度
−70°C
一般描述
This product has been discontinued.
Recombinant, 51 kDa catalytic domain of the Yersinia protein tyrosine phosphatase containing a C235R mutation and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.
外形
In 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% Brij® 35 Detergent, 50% glycerol, pH 7.0.
制备说明
Following initial thaw, aliquot and freeze (-70°C).
其他说明
One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.2.
Zhang, Z.Y., et al. 1992. J. Biol. Chem.267, 23759.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Guan, K. and Dixon, J.E. 1990. Science249, 653.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Guan, K. and Dixon, J.E. 1990. Science249, 653.
法律信息
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
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