540411
嘌呤霉素，二盐酸盐，经细胞培养测试
Puromycin, CAS 58-58-2, is a protein synthesis inhibitor that causes premature release of nascent polypeptide chains.
别名:
嘌呤霉素，二盐酸盐，经细胞培养测试
产品名称
嘌呤霉素，二盐酸盐，经细胞培养测试, Puromycin, CAS 58-58-2, is a protein synthesis inhibitor that causes premature release of nascent polypeptide chains.
InChI
1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16?,18+,22+;;/m0../s1
InChI key
MKSVFGKWZLUTTO-USYAMCSGSA-N
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
impurities
≤10 EU/mg Endotoxin (dry weight)
color
white to off-white
solubility
methanol: 20 mg/mL
water: 50 mg/mL
shipped in
ambient
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
首要靶标
蛋白质合成
蛋白质合成
Disclaimer
毒性：有害（C）
General description
一种氨基核苷类抗生素，可作为原核和真核蛋白质合成抑制剂。类似于氨酰tRNA的氨酰腺苷酸末端，竞争与大核糖体亚单位的“A位点”结合。其掺入生长中的多肽，导致链延伸终止和新生多肽基嘌呤霉素的释放。用于选择表达转染pac基因（白链霉菌）的哺乳动物细胞系，其产物嘌呤霉素乙酰转移酶通过乙酰化使药物失活。诱导胸腺细胞和HL-60白血病细胞内DNA断裂。转染哺乳动物细胞的推荐选择范围为1至30 µg/ml。
嘌呤霉素，CAS 58-58-2，是一种蛋白质合成抑制剂，可导致新生多肽链过早释放。
蛋白质合成抑制剂。通过导致新生多肽链过早释放来抑制翻译。诱导胸腺细胞和HL-60白血病细胞内DNA断裂。
Other Notes
Chow, S.C., et al. 1995.Exp.Cell Res.216, 149.
Kaufman, S.H., et al. 1993.Cancer Res.53, 3976.
de la Luna, S. and Ortín, J. 1992.Methods Enzymol.216, 376.
Chow, S.C., et al. 1991.Exp.Cell Res. 216, 149.
Kaufman, S.H., et al. 1993.Cancer Res.53, 3976.
de la Luna, S. and Ortín, J. 1992.Methods Enzymol.216, 376.
Chow, S.C., et al. 1991.Exp.Cell Res. 216, 149.
Preparation Note
复溶后，通过0.22 µm孔径的过滤器过滤灭菌，等分并冷冻（-20°C）。贮备溶液在-20°C下可稳定保存至多3个月。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
涉药品监管产品
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