biological source
mouse
antibody form
diluted ascites fluid
antibody product type
primary antibodies
clone
mab9, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, bovine, canine, rat, hamster, monkey
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RAB9A(9367)
General description
Mouse monoclonal antibody supplied as diluted ascites. Recognizes the ~26 kDa Rab9 protein.
This Anti-Rab9 Mouse mAb (mab9) is validated for use in Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Rab9.
Immunogen
recombinant, canine Rab9
Application
Immunoblotting (1:1000)
Immunofluorescence (see comments)
Immunohistochemistry (1:500)
Immunoprecipitation (see comments)
Immunofluorescence (see comments)
Immunohistochemistry (1:500)
Immunoprecipitation (see comments)
Physical form
Ascites diluted in PBS.
Other Notes
Reacts with both prenylated and non-prenylated Rab9. Does not cross-react with other Rab family members. This antibody has also been reported to work for immunofluorescence and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Schimmoller, F., et al. 1998. J. Biol. Chem.273, 22161.
Shapiro, A. and Pfeffer, S. 1995. J. Biol. Chem.270, 11085.
Pfeffer, S. 1993. Mol. Biol. Cell4, 425.
Shapiro, A. and Pfeffer, S. 1995. J. Biol. Chem.270, 11085.
Pfeffer, S. 1993. Mol. Biol. Cell4, 425.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Sang H Lee et al.
Nature communications, 9(1), 3434-3434 (2018-08-26)
Secreted Wnts play crucial roles in synaptogenesis and synapse maintenance, but endogenous factors promoting synapse elimination in central neurons remain unknown. Here we show that proline-rich 7 (PRR7) induces specific removal of excitatory synapses and acts as a Wnt inhibitor.
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