553510
RAD51-Stimulatory Compound-1, RS-1
A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis.
别名:
RAD51-Stimulatory Compound-1, RS-1, 3-((Benzylamino)sulfonyl)-4-bromo-N-(4-bromophenyl)-benzamide, RS-1
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关于此项目
经验公式（希尔记法）:
C20H16Br2N2O3S
化学文摘社编号:
分子量:
524.23
UNSPSC Code:
12352200
NACRES:
NA.51
MDL number:
Assay:
≥95% (HPLC)
Form:
solid
Storage condition:
OK to freeze, protect from light
Quality Segment
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
light beige
solubility
ethanol: 10 mg/mL, DMSO: 100 mg/mL
shipped in
ambient
storage temp.
2-8°C
SMILES string
[S](=O)(=O)(NCc3ccccc3)c1c(ccc(c1)C(=O)Nc2ccc(cc2)Br)Br
InChI
1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)
InChI key
SWKAVEUTKGKHSR-UHFFFAOYSA-N
General description
A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis. Although RS-1 enhances hRAD51 filament formation on ssDNA with or without the cofactor NTP, active filaments and recombinase activity are only induced in the presence of ATP or AMP-PNP, but not with ADP or no cofactors. Shown to promote resistance of primary human neonatal dermal fibroblasts to Cisplatin- (Cat. No. 232120) induced death in a dose-dependent manner. RS-1 is inactive toward related DNA strand exchange proteins scRAD5 and scDMC1 of yeast origin or E. coli RedA.This HDR (homology-directed repair) enhancer, is shown to significantly increase both Cas9 & TALEN-mediated knock-in efficiencies.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up 3 months at -20°C.
Other Notes
Song, J., et al. 2016. Nat. Comm., In Press.
Jayathilaka, K., et al. 2008. Proc. Natl. Acad. Sci. USA.105, 15848.
Jayathilaka, K., et al. 2008. Proc. Natl. Acad. Sci. USA.105, 15848.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable