Ribonuclease Inhibitor, Human, Recombinant, E. coli

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T₁ and S1 nuclease from Aspergillus.

Cell permeable: no

Primary Target
inactivates RNase

Product does not compete with ATP.

Reversible: no

Toxicity: Standard Handling (A)

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

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