assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥12,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
This product has been discontinued.
Recombinant, human p38α/SAPK2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified SAP Kinase in vitro with MKK6. Suitable for labeling SAP Kinase substrates.
Recombinant, human p38α/SAPK2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified SAPK2α in vitro with MKK6. Suitable for labeling SAPK2α substrates.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Guo, X., et al. 2003. J. Biol. Chem.278, 22237.
Sanz-Moreno V., et al. 2003. Mol. Cell Biol.23, 3079.
Sanz-Moreno V., et al. 2003. Mol. Cell Biol.23, 3079.
One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol of phosphate to myelin basic protein (MBP) per min at 30°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
法规信息
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Brian L Olson et al.
Genes & development, 22(2), 252-264 (2008-01-17)
Peroxisome proliferator-activated receptor gamma (PPARgamma) coactivator-1alpha (PGC-1alpha) is a highly regulated transcriptional coactivator that coordinates energy metabolism in mammals. Misregulation of PGC-1alpha has been implicated in the pathogenesis of several human diseases, including diabetes, obesity, and neurological disorders. We identified
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