biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
General description
This product has been discontinued.
Recognizes the ~17 kDa stathmin protein.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~17 kDa stathmin protein.
Immunogen
a synthetic peptide corresponding to amino acids near the C-terminus of mammalian stathmin
Application
Immunoblotting (1:20,000)
Immunohistochemistry (1:1000)
Immunoprecipitation (see comments)
Immunohistochemistry (1:1000)
Immunoprecipitation (see comments)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Does not cross-react with other cellular proteins as determined by immunoblotting. For immunoprecipitation, 1 µl of undiluted serum will immunoprecipitate approximately 20 ng of stathmin. Variables associated with assay conditions will dictate the proper working dilutions.
Koppel, J., et al. 1990. J. Biol. Chem.265, 3703.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10-13 - German Storage Class 10 to 13
法规信息
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Sangyan Chen et al.
Oncology reports, 41(3), 2051-2059 (2019-01-11)
Our previous studies demonstrated that high expression of oncoprotein 18 (Op18)/stathmin promotes malignant transformation of non‑small cell lung cancer NCI‑H1299 cells. Investigation of the cellular settings determined that NCI‑H1299 cells were genetically p53 deficient. In order to determine whether p53
Yan Zhao et al.
Oncology reports, 41(1), 501-511 (2018-10-16)
Our previous studies have identified that silencing oncoprotein 18 (Op18)/stathmin via RNA interference (RNAi) inhibited autocrine interleukin‑10 (IL‑10) and enhanced the sensitivity to Taxol in NCI‑H1299 non‑small cell lung cancer cells. In this study, whether autocrine IL‑10 regulates Op18/stathmin signaling in
Noriaki Ohkawa et al.
Journal of cell science, 120(Pt 8), 1447-1456 (2007-03-29)
The regulation of microtubule dynamics is important for the appropriate arborization of neuronal dendrites during development, which in turn is critical for the formation of functional neural networks. Here we show that stathmin, a microtubule destabilizing factor, is downregulated at