assay
≥95% (HPLC)
form
solid
color
off-white
solubility
DMF: 10 mg/mL
General description
A heterobifunctional cross-linker. Reagent similar to SATA, useful for introducing protected thiol groups into a variety of proteins. Treatment with DTT releases the chromophore 2-pyridylthione and a mercaptopropionyl-modified amine. This thiolated biomolecule can then be coupled to a second biomolecule through an iodoacetamide or maleimide group. In addition, the thiolated biomolecule can be reacted with a second pyridyldisulfide-containing biomolecule.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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D A Vallera et al.
Journal of immunological methods, 197(1-2), 69-83 (1996-10-16)
An immunotoxin consisting of a monoclonal antibody specific for CD7, a cell surface determinant expressed on T acute lymphocytic leukemia (T-ALL) blast cells, was linked to the potent plant toxin deglycosylated ricin toxin A chain (dgRTA) and is currently under
Preparation of fluorescent, cross-linking, and biotinylated calmodulin derivatives and their use in studies of calmodulin-activated phosphodiesterase and protein phosphatase.
R L Kincaid et al.
Methods in enzymology, 159, 605-626 (1988-01-01)
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